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More efficient starch processing with enzymes | Novozymes

More efficient starch processing with enzymes | Novozymes

Novozymes Secura ® is a beta-amylase for maltose production that offers temperature stability and higher, more consistent enzyme activity than plant-based alternatives. The result is simpler starch processing and the lowest cost of conversion. Using Secura ® in combination with LpHera ® allows you to run at a lower pH from liquefaction to saccharification.

Saccharification and liquefaction of cassava starch: an .

Saccharification and liquefaction of cassava starch: an .

The reaction mixture was kept at 65°C for liquefaction (15.0 minutes) and at 60°C for saccharification (90.0 minutes). The reaction was terminated by boiling the reaction mixture for 10 minutes. The volume was reduced up to 2.5 folds and the liberated glucose was detected before and after concentrating the reaction mixture.

High-Solids Enzymatic Saccharification Screening Method .

High-Solids Enzymatic Saccharification Screening Method .

May 01, 2009 · Shake flasks do not provide adequate mixing at high solids conditions. In this work, a roller bottle reactor was identified as a small-scale high-solids saccharification reaction vessel, and a method was developed for use in screening both pretreated biomass and enzyme systems at process-relevant conditions.

Enzymatic hydrolysis and saccharification optimisation of .

Enzymatic hydrolysis and saccharification optimisation of .

Effects of the stage of AMG addition Longer reaction times in the extruder were essential for efficient saccharification; in the presence of AMG, extent of saccharification was improved (both initial and subsequent) with a concomitant reduction in SME consumption (Table 6) on introducing the enzyme in zone 3 rather than zone 4 (Fig. 8a,b).

High solid and low enzyme loading based saccharification .

High solid and low enzyme loading based saccharification .

High solid and low enzyme loading based saccharification of agricultural biomass Yu Zhang, Yun-Yun Liu, Jing-Liang Xu,* Zhen-Hong Yuan,* Wei Qi, Xin-Shu Zhuang, and Min-Chao He Two agricultural biomass materials, namely wheat straw and sugarcane bagasse, were pretreated with NaOH and then used as substrates for enzymatic saccharification.

Expression of Glycosyl Hydrolases in Lignocellulosic .

Expression of Glycosyl Hydrolases in Lignocellulosic .

Ethanol produced from lignocellulosic feedstock is a promising alternative to fossil fuels and corn-sourced ethanol. However, it creates unique challenges in terms of requirements for breakdown to fermentable sugars, including the need for pretreatment and enzymatic hydrolysis of structural carbohydrates. Hydrolases from microorganisms are currently utilized for biomass hydrolysis in the .

Enzymatic Saccharification of Lignocellulosic Residues by .

Enzymatic Saccharification of Lignocellulosic Residues by .

According to Leu and Zhu, the efficiency of the enzymatic saccharification depends on factors such as the type of pretreatment of the substrate and the catalytic action of the enzymes (inhibiting effect by the final product formed, deactivation or denaturation due to reaction time, temperature, stirring and pH, synergic actuation of .

Deconstruction: Saccharification - ABPDU

Deconstruction: Saccharification - ABPDU

During saccharification, the enzyme or catalyst can be constrained by the presence of some inhibitors generated during pretreatment. The fermentation process can be combined with saccharification in an SSF process, where enzymes are applied simultaneously with the micro-organism. . reactors. Similarly, an energy balance can be established .

Studies on Enzymatic Liquefaction and Saccharification of .

Studies on Enzymatic Liquefaction and Saccharification of .

(1968). Studies on Enzymatic Liquefaction and Saccharification of Starch. Agricultural and Biological Chemistry: Vol. 32, No. 7, pp. 860-872.

Enzymatic saccharification of olive mill solid residue in .

Enzymatic saccharification of olive mill solid residue in .

The overall enzyme activity loss after a 5 h diafiltration period was 28%, however about half this loss appeared to be due to enzyme denaturation inside the reactor. During saccharification trials .

Increasing Ethanol Titer and Reducing Enzyme Dosage via .

Increasing Ethanol Titer and Reducing Enzyme Dosage via .

enzyme, 6 ml of tetracycline solution, and 100 g of kraft pulp. This provided an initial enzyme dosage of 133% and a SLR of 15%. The temperature was set to 35 . o. C instead of 50 . o. C, so the results would be more directly comparable to subsequent SSF trials. The agitation rate was set to 60 rpm, and saccharification was performed for 96 h.

Enzymatic membrane reactor for full saccharification of .

Enzymatic membrane reactor for full saccharification of .

Ultrafiltration reactors based on polymeric or ceramic membranes were shown to be suitable catalytic systems for fast enzymatic saccharification of cellulose, allowing the full recovery and reuse of enzymes.

Optimization of the enzymatic saccharification process of .

Optimization of the enzymatic saccharification process of .

Results of the effect of reaction duration on the enzymatic saccharification of the pretreated EFB showed that the reaction time more than 24 h contributed little to the rate of saccharification. The results shown in Fig. 4 indicated that after 24 h, the rate of sugar production tended to be static even with the increase in time.

Saccharification | Definition of Saccharification by .

Saccharification | Definition of Saccharification by .

Saccharification definition is - the process of breaking a complex carbohydrate (such as starch or cellulose) into its monosaccharide components.

Saccharification - an overview | ScienceDirect Topics

Saccharification - an overview | ScienceDirect Topics

In SSF, optimized temperature can be used in the saccharification process to maximize the enzymatic activity, thereby effectively producing simple sugars. The accumulation of simple sugars can inhibit the enzymes. This can lower the saccharification rate in the reactor vessel, which in turn lowers the ethanol yield in the fermentation vessel.

Demonstration-scale enzymatic saccharification of sulfite .

Demonstration-scale enzymatic saccharification of sulfite .

The saccharification reactions were started in fed-batch using Reactor 1. Substrate with approximately 30% (w/w) DM was gradually fed into the reactor containing enzymes and SSL, to a targeted final working weight of approximately 4000 kg, substrate loading of 12% (w/w) DM pulp and enzyme loading of 4% (w liquid/w DM of substrate).

Continuous Saccharification of Pretreated Lignocellulose .

Continuous Saccharification of Pretreated Lignocellulose .

Continuous Saccharification of Pretreated Lignocellulose in Simulated Moving Bed Reactor. . Conversion of cellulose to glucose is a complex process and its kinetics is governed by a number of substrate and enzyme related factors. In theory, continuous removal of the glucose minimizes the product inhibition on the enzymes which in turn leads .

Hydrothermal treatment and enzymatic saccharification of .

Hydrothermal treatment and enzymatic saccharification of .

Full Article. Hydrothermal Treatment and Enzymatic Saccharification of Corncobs. Tang-Sheng Sun, a Kun Wang, a Guihua Yang, b Hai-Yan Yang, a and Feng Xu* a Hydrothermal pretreatment of corncobs in aqueous media under non-isothermal conditions is an effective means for solubilizing hemicellulose fractions and improving cellulose hydrolysis.

The Starch Conversion/Saccharification Rest - How to Brew

The Starch Conversion/Saccharification Rest - How to Brew

The Starch Conversion/Saccharification Rest. Finally we come to the main event: making sugar from the starch reserves. In this regime the diastatic enzymes start acting on the starches, breaking them up into sugars (hence the term saccharification). The amylases are enzymes that work by hydrolyzing the straight chain bonds between the .

Kinetic modeling of countercurrent saccharification .

Kinetic modeling of countercurrent saccharification .

Jul 11, 2019 · Countercurrent saccharification is a promising way to minimize enzyme loading while obtaining high conversions and product concentrations. However, in countercurrent saccharification experiments, 3–4 months are usually required to acquire a single steady-state data point. To save labor and time, simulation of this process is necessary to test various reaction conditions and determine the .

Laboratory-scale method for enzymatic saccharification of .

Laboratory-scale method for enzymatic saccharification of .

This comparison assessed the reaction systems for their efficiency and repeatability in converting biomass at high-solids loadings, where biomass conversion was the measure of effectiveness of enzymatic saccharification. When biomass and enzyme are effectively mixed, yield is similar, regardless of reactor system.

Continuous Saccharification of Corn Starch in a Membrane .

Continuous Saccharification of Corn Starch in a Membrane .

Continuous Saccharification of Corn Starch in a Membrane Reactor. Part II: Membrane Performance and Reactor Stability . At the enzyme concentrations employed in these studies, loss of enzyme activity due to membrane leakage, mechanical shear, thermal inactivation and/or membrane "poisoning" had little effect on reactor stability and .

Cellulosic ethanol production system Energy creation from .

Cellulosic ethanol production system Energy creation from .

in the back and front) to keep the reaction time constant. This reactor also comes with a self-cleaning function. The saccharification reactor used in the component test facility is shown in Fig. 7. Fig. 7 Saccharification reactor used for the demonstration test in Akita Table 3 Transition of saccharification reactor Item NEDO joint study Akita .

Hydrolysis - Wikipedia

Hydrolysis - Wikipedia

Saccharification reaction processes were studied using three reactor types: batch, continuous stirred tank reactor (CSTR), and the tubular plug flow process. The plug flow process produced the most favorable results in that large quantities of free enzyme were recoverable from this process at late stages of the reaction.

US4409329A - Saccharification method - Google Patents

US4409329A - Saccharification method - Google Patents

US4409329A US06/398,618 US39861882A US4409329A US 4409329 A US4409329 A US 4409329A US 39861882 A US39861882 A US 39861882A US 4409329 A US4409329 A US 4409329A Authority US United States Prior art keywords cellulose accordance method concentration enzyme complex Prior art date 1981-03-23 Legal status (The legal status is an assumption and is not a legal conclusion.

3.5 Alcohol production using an integrated pilot plant

3.5 Alcohol production using an integrated pilot plant

Saccharification from both low- and high-concentration biomass was investigated, and an appraisal of the most suitable types of reactor was made. Owing to the high cost of cellulase, its recovery and re-use were investigated using UF techniques.

SACCHARIFICATION REACTION MIXTURE, SACCHARIFICATION ENZYME .

SACCHARIFICATION REACTION MIXTURE, SACCHARIFICATION ENZYME .

Oct 03, 2019 · 1. A saccharification reaction mixture, wherein the reaction mixture can saccharify at least one of cellulose and hemicellulose and comprises at least one of cellulose and hemicellulose, a saccharification enzyme, silica or a silica-containing substance, and at least one compound (A) selected from the group consisting of a compound represented by the following formula (1) or (2): .

Saccharification of potato starch in an ultrafiltration .

Saccharification of potato starch in an ultrafiltration .

The overall enzyme activity loss after a 5 h diafiltration period was 28%, however about half this loss appeared to be due to enzyme denaturation inside the reactor. During saccharification trials .

Enzymatic saccharification of pretreated corn stover in a .

Enzymatic saccharification of pretreated corn stover in a .

Dec 18, 2010 · Enzymatic hydrolysis of corn stover was performed in an integrated membrane bioreactor (MBR) incorporating a 10 kDa flat sheet polysulfone membrane to increase cellulose conversion and to reduce enzyme dosage. Several pretreatment methods and semi-continuous MBR were examined to investigate their effect on the glucose yield and enzyme utilization efficiency.

Enzymatic saccharification of cellulose: a study of mixing .

Enzymatic saccharification of cellulose: a study of mixing .

Cellulose saccharification has been shown to be a function of agitation. Here, the effect of agitation by oscillatory mixing in an oscillatory baffled reactor (OBR) has been assessed and contrasted with a stirred tank reactor (STR). After 168 h of saccharification at 200 Wm−3, 91% conversion of the cellulose (∼25 g/L glucose) was observed in the OBR, as against 74% conversion (∼21 g/L .